Co-Lecturing Between Research Center for Detection of Pathogenic Bacteria LPPM UNJ and the National Kaohsiung University of Science and Technology (NKUST) #2

Jakarta - Tuesday, November 21'st, 2023. Research Center for Detection of Pathogenic Bacteria LPPM UNJ held a Co-Lecturing Between Research Center for Detection of Pathogenic Bacteria LPPM UNJ and the National Kaohsiung University of Science and Technology (NKUST) with the theme “Environment, Nanotechnology, and Biotechnology”. This Co-lecturing is a continue session of the activity in the previous week on November 14'th, 2023. This activity showed the presentation of 4 interesting research materials conducted by Research Center for Detection of Pathogenic Bacteria LPPM UNJ, namely the Development of Listeria monocytogenes Detection Kit by Real-Time PCR and Sampling and Efficacy Test of Listeria monocytogenes Master Diagnostic Detection Kit, as well as research conducted by NKUST, namely Soil Biotechnology for Toxin Removal and Bioremediation of Trichloroethylene Contaminated Groundwater.

The first presentation was presented by Prof. Shyi-Tien Chen who is a Professor in the Department of Safety, Health, and Environmental Engineering, NKUST. He talked about his research and the title is Soil Biotechnology for Toxin Removal or the application of biotechnology in removing toxins in soil. The discussion of this material began with an explanation of the structure of the earth and events that affect changes it. Then, the explanation about soil remediation technology, sources of contamination, characteristics of contaminant, and also the mechanism of t-RFLP as a solution for toxin removal are discuss by him in this session. In this study, he also monitored the degradation efficiency of PCP and PAHs at two stages of treatment.

The second material was presented by Jefferson Lynford Declan, S.Si. who is a research team at Research Center for Detection of Pathogenic Bacteria LPPM UNJ. He explained about the Development of Listeria monocytogenes Detection Kit by Real-Time PCR. He targeted the inlA virulence gene to detect contamination of L. monocytogenes in vegetables because it can mediate the entry of L. monocytogenes into the host's intestinal epithelial cells and cause food poisoning. He said the inlA primer can detect L. monocytogenes contamination in lettuce and cucumber and the primer also can distingusih the target and non-target bacteria based on the Ct and the Melting Curve using rt-PCR.

The third presentation was presented by Jason Shen who is a Ph.D. candidate and research team from the National Kaohsiung University of Science and Technology (NKUST). He presented about the Bioremediation of Trichloroethylene Contaminated Groundwater based on the current situation in the environment. The presentation begin with an explanation of the geological and hydrological properties of the environment such as groundwater flow, hydraulic conductivity, groundwater level, and the condition of clay, fill, fine sand, and mud. Later, he also explained the method use for bioremediation of trichloroethylene-contaminated groundwater to enhance in-situ bioremediation while keep considering the TCE pollution distribution map and adjusting both zone and strategy of the pollution.

The last material was presented by Tiara Fahriza, who is a research team of Research Center for Detection of Pathogenic Bacteria LPPM UNJ. She explained about Sampling and Efficacy Test of Listeria monocytogenes Master Diagnostic Detection Kit. She said, the efficacy test conducted to evaluate the accuration of the prototype in detect L. monocytogenes on food samples by comparing the culture, PCR, and real-time PCR method. Based on the data, food samples show positive results of L. monocytogenes by testing all the three methods.

The activity continued with a discussion and QnA session guided by Anisa Fitriyanti and Gusti  Angieta Putri as a Moderator. This Co-Lecturing was held online through the Zoom Cloud Meeting and live on the Youtube channel PUI Pendeteksi Bakteri Patogen LPPM UNJ. This activity finished at 10.30 GMT+7 .

(aips/gap)

*This event is partial funded by the NKUST via an international project funded by Ministry of Education, Taiwan.*